Journal: Endocrinology

Article Title: Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex.

doi: 10.1210/en.2004-0179

Figure Lengend Snippet: FIG. 5. Effect of Dex treatment on adrenal cortex vasculature and VEGF expression. Sections of adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14) were immunostained for CD31 (A) or VEGF (B). The dotted lines indicate the border between adrenal cortex and medulla. Immunoreactivity is indicated by brown staining. Data from one representative experiment are shown. Similar results were obtained in three independent experiments.

Article Snippet: Slides were then incubated for 1 h with 0.5 g/ml rabbit polyclonal antihuman VEGF antiserum (A20; Santa Cruz Biotechnology, Santa Cruz, CA), which recognizes all VEGF isoforms or with a rat anti-BrdU at a dilution of 1/75 (clone BU1-75; Harlan, Indianapolis, IN).

Techniques: Expressing, Staining

Journal: Endocrinology

Article Title: Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex.

doi: 10.1210/en.2004-0179

Figure Lengend Snippet: FIG. 6. Effects of Dex treatment on the expression of endocrine and endothelial cell markers. A, RT-PCR analysis of the expression of VEGF-A isoforms (the two bands correspond to specific amplification products of the VEGF120 and VEGF164 isoforms), VEGF-Rs (VEGF-R1/flt-1, VEGF-R2/flk-1, and neuropilin-1), endocrine cell markers (ACTH receptor/MC2-R, HDL receptor/SR-B1), and endothelial cell markers (PECAM and VE-cadherin) in the adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14). RT-PCR amplification of HPRT mRNA was used as an internal standard for the normalization of the samples. The results from one representative experiment are shown. Similar results were obtained in three independent experiments. B and C, Quantitative determination of adrenal VEGF-A and VEGF-Rs mRNA levels by real-time RT-PCR was performed as described in Materials and Methods. The ratio of level of expression of the gene of interest to that of GAPDH was normalized to 1 in control adrenals. The relative quantities of the following mRNAs were plotted as a function of time of Dex treatment: VEGF-A (B); VEGF-R1 (C, solid line), VEGF-R2 (C, continuous dotted line), and neuropilin-1 (C, discontinuous dotted line). Each value represents the mean SEM from 10 to 15 mouse adrenals collected in three independent experiments.

Article Snippet: Slides were then incubated for 1 h with 0.5 g/ml rabbit polyclonal antihuman VEGF antiserum (A20; Santa Cruz Biotechnology, Santa Cruz, CA), which recognizes all VEGF isoforms or with a rat anti-BrdU at a dilution of 1/75 (clone BU1-75; Harlan, Indianapolis, IN).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Quantitative RT-PCR, Control

Journal: Nature cardiovascular research

Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

doi: 10.1038/s44161-022-00138-1

Figure Lengend Snippet: Fig. 1 | Single-cell and spatial transcriptomics of cardiac and ileum tissue of reovirus-infected neonatal mice. a, Experiment and analysis workflow. Four- day-old neonatal mice weighing 3 g per pup were infected (per os) with reovirus T1L. Neonatal mice infected with 1× PBS were used as mock controls. Ileum tissue (1 dpi and 4 dpi) and heart tissues (4 dpi, 7 dpi and 10 dpi) were assayed and used for scRNA-seq and spatial transcriptomics. b, UMAP plot of 31,684 single-cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi (one animal per condition), clustered by gene expression and colored by cell type (left). UMAP plots showing cardiac cell type clusters across samples for the heart scRNA-seq data (right). c, 8,243 spatial transcriptomes of cardiac tissue sections from mock-infected and reovirus-infected mice at 4 dpi and 7 dpi (one animal per condition). H&E-stained image of reovirus-infected myocarditic

Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

Techniques: Infection, Gene Expression, Staining

Journal: Nature cardiovascular research

Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

doi: 10.1038/s44161-022-00138-1

Figure Lengend Snippet: Fig. 3 | Cytotoxic T cells recruited by inflamed endothelial cells induce pyroptosis in myocarditic tissue. a, UMAP plot of 9,786 single-cell endothelial cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by endothelial cell subtype clusters (phenotypes) (top) and condition (bottom). b, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for endothelial cell subtypes. c, UMAP plot showing the expression of genes upregulated in Cxcl9-high endothelial cells. d, Spatial transcriptomic maps of cardiac tissue from reovirus infected hearts at 7 dpi showing gene module scores calculated for four GO terms enriched in Cxcl9-high endothelial cells. e, UMAP plot of 2,205 single-cell T cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by T cell subtype clusters (top) and condition (bottom). f, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for T cell subtypes. g, UMAP plot showing the expression of genes upregulated in cytotoxic T cells from myocarditic heart at 7 dpi. h, Spatial transcriptomics maps of cardiac tissue from reovirus infected hearts at 7 dpi

Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

Techniques: Infection, Expressing

Journal: Nature cardiovascular research

Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

doi: 10.1038/s44161-022-00138-1

Figure Lengend Snippet: Fig. 4 | Myocarditic regions and the border zone have distinct transcriptomic profiles and cell-type-specific signatures. a, Spatial transcriptomics map of cardiac tissue section from reovirus-infected mice at 7 dpi colored by spot clusters representing transcriptionally distinct tissue regions. b, Spatial transcriptomics maps of cardiac tissue sections from reovirus-infected mice at 7 dpi showing the expression of differentially expressed genes of interest in the myocarditic and the border zone. c, Changes in average predicted cell type proportions across the infected ventricle, for cell types enriched in the myocarditic region and the border zone. d, UMAP plot of 502 single-cell cardiomyocyte cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by myocyte cell subtype (phenotypes) (left) and condition (right). e, Heat map showing the top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for cardiomyocyte cell subtypes. f, Venn diagram showing myocyte-specific genes

Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

Techniques: Infection, Expressing

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: A) Heatmap showing OR expression in dermis, epidermis and tape strip samples from AD patients (L=lesional, NL=non-lesional) and normal controls (NC). The highest expression of olfactory receptors was found in skin tape strip samples. The expression of OR10G7 receptor followed in this manuscript is outlined on the heatmap. B) Expression of OR10G7 mRNA in skin tape strip samples of AD patients and healthy controls as detected by RNAseq analysis.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Expressing, Stripping Membranes

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: A) OR10G7 and B) FLG-1 expression in matched skin biopsies of normal controls (NC) and AD patients are shown by arrows. OR10G7/FLG-1 – cy3, red. Wheat germ agglutinin (FITC, green) was used to counter stain cell membranes. Magnification 200x.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Expressing, Staining

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: A) OR10G7 mRNA and B) FLG-1 mRNA expression were measured by real time PCR (RTPCR) in AD non-lesional (AD) and normal control (NC) skin biopsies. There is increased OR10G7 mRNA expression in AD non-lesional skin compared to NC skin (p=0.03), with corresponding reduced FLG-1 mRNA expression in AD non-lesional skin compared to NC skin (p=0.001). A two-tailed t-test was used for significance testing. Data are shown as the mean ± SD.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: Primary human keratinocytes from NC and AD patients were grown in culture and harvested in the undifferentiated state, Day 1 and Day 4 post 1.3mM CaCl2 differentiation. OR10G7 (A) and FLG-1 (B) mRNA expression in undifferentiated and differentiated keratinocytes is shown. OR10G7 mRNA expression is higher in AD than NC keratinocytes at all stages of Ca2+ differentiation (p<0.05). OR10G7 expression is also down-regulated with progressive Ca2+ differentiation in both AD and NC keratinocytes (p<0.05). FLG-1 mRNA expression was lower in AD than NC keratinocytes at all stages of Ca2+ differentiation, particularly on Day 4 post differentiation (p<0.05). Each condition was performed in triplicate, n=3 experiments. A two-tailed t-test was used for significance testing. Data are shown as the mean ± SD.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Expressing, Two Tailed Test

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: Control or OR10G7 siRNA transfected keratinocytes were treated with either media (control) or varying concentrations of A) acetophenone or B) eugenol. Production of ATP was measured using the CellTiter-Glo® Assay and luminescence units (RLU) recorded using a luminometer after 2 minutes incubation.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Transfection, Glo Assay, Incubation

Journal: The Journal of allergy and clinical immunology

Article Title: The Expression and Function of the Ectopic Olfactory Receptor OR10G7 in Atopic Dermatitis

doi: 10.1016/j.jaci.2018.11.004

Figure Lengend Snippet: Control or OR10G7 siRNA transfected keratinocytes were treated with either media (control) or varying concentrations of A) acetophenone or B) eugenol and IL-1β mRNA expression measured using RT-PCR. A two-tailed t test was used for significance testing. Data are shown as the mean ± SD.

Article Snippet: Slides were then stained with a rabbit polyclonal anti-OR10G7 antibody (1:500 dilution; OriGene Technologies, Rockville, MD) or a rabbit polyclonal anti-FLG-1 antibody (1:500 dilution, BioLegend, San Diego, CA) or equal amount of rabbit IgG as an isotype control at 4°C overnight.

Techniques: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test